An Atypical Form of αB-crystallin Is Present in High Concentration in Some Human Cataractous Lenses
نویسندگان
چکیده
منابع مشابه
Existence of deamidated alphaB-crystallin fragments in normal and cataractous human lenses.
PURPOSE The aims of this study were to characterize lens crystallin fragments having a molecular mass of <10 kDa, isolated by solubilization in trichloroacetic acid, in order to identify cleavage sites in the parent crystallins for their origin and determine post-translational modifications in the fragments. METHODS The water-soluble (WS) and water-insoluble (WI) protein fractions were isolat...
متن کاملDiscrepant expression of microRNAs in transparent and cataractous human lenses.
PURPOSE Age-related cataracts are considered to be a pathological condition that arise as senescence progresses. However, little is known about the function of microRNAs (miRNAs) in the formation of age-related cataracts. The purpose of this study was to identify possible differences in miRNA expression in the central epithelium of transparent and age-related cataractous human lenses. METHODS...
متن کاملNicotinamide Nucleotides in Different Types of Human Cataractous Lenses
Human Cataractous Lenses T a n w e e r Sarwat , M. A tau r Rahman* , and T. H. Kirmani b Departments of Biochemistry" and Ophthalmology. Jinnah Postgraduate Medical Centre. Karachi. Pakistan Z. Naturforsch. 43b, 1056-1058 (1988); received February 19. 1988 Human Cataractous Lenses, Nicotinamide Nucleotides Ratio, Senile Cataracts The ratios of reduced and oxidized nicotinamide nucleotides (NADH...
متن کاملLoss of thiol repair systems in human cataractous lenses.
PURPOSE The purpose of this study was to investigate the thiol repair systems of thioltransferase (TTase) and thioredoxin (Trx) and oxidation-damaged proteins in human cataractous lenses. METHODS Cataractous lenses in humans (57-85 years of age) were classified into cortical, nuclear, mixed, mature, and hypermature cataract types by using a lens opacity classification system, and were obtaine...
متن کاملFree and bound water in normal and cataractous human lenses.
PURPOSE To analyze free and total water in human normal and cataractous lenses. METHODS Thermogravimetric analysis was used to determine total water, and differential scanning calorimetry was used for free water. RESULTS In normal human lenses, the total water content of the nucleus remained unchanged with age, but the state of the water altered. The ratio of free to bound water increased s...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1999
ISSN: 0021-9258
DOI: 10.1074/jbc.274.45.32287